郜卫华,谭北平,麦康森,迟淑艳,刘泓宇,董晓慧,杨奇慧

• 1:长江大学动物科学学院水产系
• 2:广东海洋大学水产学院水产动物营养与饲料实验室
• 3:中国海洋大学教育部海水养殖重点实验室

摘要(Abstract)：

本研究构建了长期低渗胁迫诱导凡纳滨对虾肝胰腺差异表达基因的cDNA文库。在此基础上,探讨了在不同盐度处理时反向文库5个基因(血蓝蛋白、蜕皮类固醇调节蛋白、几丁质酶、胰凝乳蛋白酶1和胰蛋白酶)mRNA表达量的变化情况,以期从分子机理上了解,在长期低盐养殖条件下凡纳滨对虾的生长性能和生理状态的关系。长期低盐养殖实验结果表明,随着盐度的降低,凡纳滨对虾的末重、增重率和特定生长率显著地降低(P<0.05);盐度2处理组成活率显著低于盐度10处理组和对照组(P<0.05);与对照组相比,盐度2处理组血蓝蛋白、几丁质酶、蜕皮类固醇调节蛋白、胰蛋白酶和胰凝乳蛋白酶1mRNA表达量显著性下调,分别下降了50%、31%、91%、25%和35%;盐度10处理组血蓝蛋白、胰蛋白酶和胰凝乳蛋白酶mRNA表达量显著性下调,分别下降了31%、72%和15%;短期低盐胁迫组几丁质酶和蜕皮类固醇调节蛋白mRNA表达量上调3.07和2.47倍。结果表明,盐度降低到一定程度,可显著降低凡纳滨对虾的生长性能和成活率;5个基因表达量在胁迫24h和56d后表达量变化明显不同,说明凡纳滨对虾对长期和短期盐度胁迫采取不同的应答策略。以上结果丰富了甲壳动物环境胁迫研究的基因信息。

关键词(KeyWords)： 凡纳滨对虾;肝胰脏;盐度;胁迫;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30871928);; 广东省高等学校珠江学者特聘教授基金项目(GDUPS-2011);; 公益性行业(农业)科研专项基金项目(201003020)资助

作者(Author): 郜卫华,谭北平,麦康森,迟淑艳,刘泓宇,董晓慧,杨奇慧

DOI: 10.16441/j.cnki.hdxb.2013.09.006

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