加氨碱化提取竹荚鱼鱼肉蛋白技术初探Tentative Development of an Ammonium-Alkalifying Technique of Trachurus Flesh Protein Extraction
赵硕,廖鄂,齐祥明,林洪
摘要(Abstract):
为探索加氨碱化提取鱼肉蛋白技术理论,本文首先以竹荚鱼肉为对象进行了鱼肉蛋白溶解基本规律的探索,其次以竹荚鱼加工副产物为对象考察了pH、碱种类、温度、料水比等因素对鱼肉蛋白提取过程的影响。结果表明:酸碱范围内分别存在一个蛋白溶解极高值区域,pH=10.0~12.0的碱性条件下蛋白溶解性较pH=2.0~3.0酸性条件更佳,pH=5.0~6.0时,鱼肉蛋白溶解性最低。鱼肉蛋白的碱溶pH区间内,温度适当提高可促进蛋白的提取,用水量适当提高有助于蛋白提取,但料水比低于1∶2后,蛋白提取总量几乎不变。当温度40℃、pH=10.5、料水比1∶2时,可获得最佳提取效果,蛋白提取总量达122.64mg/g。料水比为1∶0.5时采用两级提取获得与上述最佳提取效果接近的结果。作为一种低成本、易操作、无残留的绿色提取技术,加氨碱化法有较好的发展前景。
关键词(KeyWords): 氢氧化钠;氨;鱼肉蛋白;鱼加工副产品;溶解性
基金项目(Foundation): 国家自然科学基金项目(31101380)资助
作者(Author): 赵硕,廖鄂,齐祥明,林洪
DOI: 10.16441/j.cnki.hdxb.2013.05.009
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