滕哈燕,张振忠,汝少国,王军

• 1:中国海洋大学海洋生命学院

摘要(Abstract)：

为快速检测海洋环境雌激素，本研究利用20 nm胶体金颗粒和褐牙鲆(Paralichthys olivaceus) Vtg单/多克隆抗体开发了夹心法免疫胶体金检测试纸条，并对胶体金标记条件、T线和C线抗体浓度等参数进行了优化，构建了褐牙鲆Vtg的快速检测技术。实验结果表明该胶体金试纸条工作范围为15.7～1 000 ng·mL~(-1)(R~2>0.98),检出限为7.8 ng·mL~(-1)。本研究制备的胶体金试纸条检出限与目前常用的多种Vtg的ELISA方法相近，但是检测时间仅为15 min,比常规方法节省了约14 h。此外，该试纸条在存放30和90 d后，对Vtg的检测结果并无明显偏差，表现出较好的稳定性和可重复性。最后，通过测定多种常见环境雌激素(双酚A、雌二醇、乙炔雌二醇)暴露对雄性成年褐牙鲆血浆中Vtg的诱导情况，进一步验证了该试纸条在海洋环境雌激素活性检测中的应用。

关键词(KeyWords)： 褐牙鲆;生物标志物;卵黄原蛋白;免疫胶体金试纸条;雌激素检测

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21607144)资助~~

作者(Author): 滕哈燕,张振忠,汝少国,王军

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