镉对中华绒螯蟹肝R—细胞亚显微结构及血清谷丙转氨酶(SGPT)活力的影响EFFECT OF CADMIUM ON ULTRASTRUCTURE OF R-CELL IN HEPATOPANCREAS AND SERUM GLUTAMIC-PYRUVIC TRANSAMINASES (SGPT) ACTIVITY OF ERIOCHEIR SINENSIS
卢敬让,赖伟,堵南山
摘要(Abstract):
作者提出镉对中华绒螯蟹肝胰脏R—细胞亚显微结构和血清谷丙转氨酶活力影响。在亚显微结构水平上,R—细胞中,受影响变化最明显的是线粒体;线粒体体积和其内膜嵴的破坏程度与介质镉浓度成显著的正相关关系;随镉浓度的升高,细胞器减少,内质网膨胀,细胞质和线粒体基质变稀薄。SGPT活力也随镉浓度升高而显著增大,并与R—细胞亚显微结构变化程度一致。本文还就处于不同镉浓度下的标本,与生理机能相联系的R—细胞形态变化的意义、R-细胞线粒体亚显微结构变化与SGPT活力变化的关系及SGPT活性变化的特点等进行了初步探讨。
关键词(KeyWords): 中华绒螯蟹;肝胰脏;血清谷丙转氨酶;镉;亚显微结构
基金项目(Foundation):
作者(Author): 卢敬让,赖伟,堵南山
DOI: 10.16441/j.cnki.hdxb.1989.s2.009
参考文献(References):
- [1] 刘志强,汞、钴对非洲鲫鱼线粒体影响的初步研究,海洋科学,1985,9(4) :36-38
- [2] Bark. P. L. et al, 1978. Observations on the structure of the mouthparts, histology of the alimentary tract and digestive physiology of the mud crab Scylla serrata (Forskol) (Decapoda: Portunidae) J of Experi Mar, Biol. 32: 177-196.
- [3] Bergmeyer, H. V. et al., 1963. Methods of enzymatic analysis. pp. 837.
- [4] Bubel, A. 1976. Histological and electron microscopical observations on the effects of different salinities and heavy metal ions on the gills of Jera nordmanni (Rathke) (Crustacea. Isopoda) Cell and Tissue Res 167: 65-95.
- [5] Bunt. A. H. 1968. An ultrastructural study of the hepatopancreas of procambarus clarkii (Girard) (Decapoda: Astacidea) Crustaceana. 15: 282-288
- [6] Corner, E. D. S. & Sparrow, B. W. 1956. The modes of action of toxic agents. I. Observations on the poisonmg of certain crustaceans by copper and mercury. J. mar. biol. Ass.. U. K. 35: 531-548
- [7] Green, D. E. et al.. 1968. Conformational basis of energy transformations in membrane systems 3 Configurational changes in functional states. Arch. Biochem. Biophys. 125: 684-705
- [8] Jennings. J. R. et al.. 1979. Studies on the uptake of cadmium by the crab carcinus maenas in the laboratory. 2. Preliminary investigation of cadmium-binding proteins. Mar. biol. 50: 141-149
- [9] Jeremy, T. P. Baker, 1969. Histological and electron microscopical observation on copper poisoning in the winter flounder (Pseudopleuronectes americanus). J. Fish. Res Board of Canada 26 (11 ): 2785-2793
- [10] Stoner, C. D. and Sirak, H. D. 1969. Osmotically-induced alterations in volume and ultrastructure of mitochondria isolated from rat liver and bovine heart Jour. of Cell Biol. Vol. 43: 521-536
- [11] Versteeg, D. J. and Giesy. J. P. 1986. The histological and biochem cal effects of cadmium exposure in the bluegill sunfish (Lepis macrochirus) Ecotoxi and Environ Safety 11: 31-43
- [12] Weible, E. R. et al.. 1966. Practical stereological methods for morphometric cytology Jour. Cell Biol. 30: 23-38
- [13] Zelman, M D.et al., 1959 Transaminase in serum and liver correlated with liver cell necrosis in needle aspiration biopsies American Jour of the Medi. Sciences. 15: 323-333
文章评论(Comment):
|
||||||||||||||||||
|
||||||||||||||||||