张胜东,王勋功,甄毓

• 1:中国海洋大学海洋环境与生态教育部重点实验室
• 2:青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室
• 3:中国海洋大学环境科学与工程学院

摘要(Abstract)：

本研究在青岛金沙滩潮上带连续采集浅层地下水样品,运用高通量测序技术及荧光定量PCR技术,探讨潮汐作用对滨海浅层地下水中细菌群落结构的影响。结果表明,滨海浅层地下水中的细菌优势类群为变形菌,潮汐作用会引起地下水中细菌群落组成与丰度发生明显变化。通过对各细菌样本的聚类分析发现,涨潮前期的样品之间、退潮阶段的样品之间相似性较高,而高潮阶段样品与它们的差异性较大。NO~-_2、SiO■对浅层地下水中的细菌群落结构具有显著影响(P<0.05)。受潮汐作用影响,浅层地下水环境中与氮循环相关的细菌群落相对丰度较高。本研究表明,潮汐作用对浅层地下水中细菌群落结构的影响不可忽视。本研究为滨海浅层地下水中微生物群落结构的研究提供了可靠依据。

关键词(KeyWords)： 潮汐作用;微生物群落;浅层地下水;高通量测序

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(U1906210);; 青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室青年人才培育项目(LMEES-YTSP-2018-02-06);; 中央高校基本科研业务项目(201962003)资助~~

作者(Author): 张胜东,王勋功,甄毓

DOI: 10.16441/j.cnki.hdxb.20200289

参考文献(References)：

• [1] Burnett W C,Bokuniewicz H J,Huettel M,et al.Groundwater and pore water inputs to the coastal zone[J].Biogeochemistry,2003,66(1):3-33.
• [2] Moore W S.The effect of submarine groundwater discharge on the ocean[J].Annual Review of Marine Science,2010,2(1):59-88.
• [3] Burnett W C,Aggarwal P K,Aureli A,et al.Quantifying submarine groundwater discharge in the coastal zone via multiple methods[J].Science of the Total Environment,2006,367(2):498-543.
• [4] Gibbes B,Robinson C,Li L,et al.Tidally driven pore water exchange within offshore intertidal sandbanks:Part II numerical simulations[J].Estuarine Coastal and Shelf Science,2008,80(4):472-482.
• [5] Anschutz P,Smith T,Mouret A,et al.Tidal sands as biogeochemical reactors[J].Estuarine,Coastal and Shelf Science,2009,84(1):84-90.
• [6] Santos I R,Eyre B D,Huettel M.The driving forces of porewater and groundwater flow in permeable coastal sediments:A review[J].Estuarine Coastal and Shelf Science,2012,98:1-15.
• [7] 刘陆.黄河口潮间带泥滩环境微生物群落特征研究[D].青岛：中国海洋大学，2014.Liu L.The Study on the Characteristics of Microbial Community in Intertidal Mudflat of the Yellow River Estuary[D].Qingdao:Ocean University of China,2014.
• [8] 刘志媛.黄河河口过程中碳的行为变化[D].青岛：中国海洋大学，2011.Liu Z Y.The Changing Behavior of Carbon From Estuarine Dynamic in Yellow River Estuary[D].Qingdao:Ocean University of China,2011.
• [9] Rogers D R,Casciotti K L.Abundance and diversity of archaeal ammonia oxidizers in a coastal groundwater system[J].Applied and Environmental Microbiology,2010,76(24):7938-7948.
• [10] Edmonds J W,Weston N B,Joye S B,et al.Microbial community response to seawater amendment in low-salinity tidal sediments[J].Microbial Ecology,2009,58(3):558-568.
• [11] Falkowski P G,Fenchel T,Delong E F.The microbial engines that drive Earth′s biogeochemical cycles[J].Science,2008,320(5879):1034-1039.
• [12] Chen J,Taniguchi M,Liu G,et al.Nitrate pollution of groundwater in the Yellow River Delta,China[J].Hydrogeology Journal,2007,15(8):1605-1614.
• [13] Moran S B,Stachelhaus S L,Kelly R P,et al.Submarine groundwater discharge as a source of dissolved inorganic nitrogen and phosphorus to coastal ponds of Southern Rhode Island[J].Estuaries and Coasts,2014,37(1):104-118.
• [14] Zheng Y,Hou L,Liu M,et al.Tidal pumping facilitates dissimilatory nitrate reduction in intertidal marshes[J].Scientific Reports,2016,6(1):1-12.
• [15] 侯贯云，翟水晶，乐晓青，等.潮汐对闽江口感潮湿地孔隙水及土壤中硅，氮浓度的影响[J].应用生态学报，2017,28(1):337-344.Hou G Y,Zhai S J,Le X Q,et al.Influences of tide on silicon and nitrogen contents in soil and porewater in the Minjiang River estuary,Southeast China[J].Chinese Journal of Applied Ecology,2017,28(1):337-344.
• [16] Cao D,Shi F,Koike T,et al.Halophyte plant communities affecting enzyme activity and microbes in saline soils of the Yellow River Delta in China[J].Clean-Soil Air Water,2014,42(10):1433-1440.
• [17] Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
• [18] Bokulich N A,Subramanian S,Faith J J,et al.Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J].Nature Methods,2013,10(1):57-59.
• [19] DeLong E F,Preston C M,Mincer T,et al.Community genomics among stratified microbial assemblages in the ocean′s interior[J].Science,2006,311(5760):496-503.
• [20] Novikova O D,Khomenko V A,Frolova G M,et al.Porins from marine bacteria of the genus Pseudoalteromonas (Gammaproteobacteria:Pseudoalteromonadaceae)[J].Russian Journal of Marine Biology,2013,39(1):58-64.
• [21] Yada S,Wang Y,Zou Y,et al.Isolation and characterization of two groups of novel marine bacteria producing violacein[J].Marine Biotechnology,2008,10(2):128-132.
• [22] G?rtner A,Wiese J,Imhoff J F.Amphritea atlantica gen.nov.sp.nov.,a gammaproteobacterium from the Logatchev hydrothermal vent field[J].International Journal of Systematic and Evolutionary Microbiology,2008,58(1):34-39.
• [23] Su J,Zhou Y,Lai Q,et al.Sinobacterium caligoides gen.nov.sp.nov.,a new member of the family Oceanospirillaceae isolated from the South China Sea,and emended description of Amphritea japonica[J].International Journal of Systematic and Evolutionary Microbiology,2013,63(6):2095-2100.
• [24] Green R T,Todd J D,Johnston A W B.Manganese uptake in marine bacteria;the novel MntX transporter is widespread in Roseobacters,Vibrios,Alteromonadales and the SAR11 and SAR116 clades[J].The ISME Journal,2013,7(3):581-591.
• [25] Guasto J S,Rusconi R,Stocker R.Fluid mechanics of planktonic microorganisms[J].Annual Review of Fluid Mechanics,2012,44:373-400.
• [26] Trick T,Custodio E.Hydrodynamic characteristics of the western Doňana region (area of El Abalario),Huelva,Spain[J].Hydrogeology Journal,2004,12(3):321-335.

文章评论(Comment)：