陈钊,罗强,常志强

• 1:中国水产科学研究院黄海水产研究所农业部海水养殖病害防治重点实验室
• 2:中国海洋大学水产学院
• 3:上海海洋大学水产与生命学院
• 4:青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室

摘要(Abstract)：

研究了14和17℃2种不同养殖温度下,氟苯尼考在健康大菱鲆(Scophthalmus maximus)体内的药代动力学特征。以15mg/kg BW的剂量经静注和口灌2种方式给药后,于0.25、0.5、1、2、4、6、8、12、16、24、48和96h采集血液样品,采用Agilent1200型高效液相色谱仪测定样品中药物的含量,药时数据采用DAS2.0自动药动学软件进行分析。研究显示,14和17℃下,静注给药后的消除相半衰期(t1/2β)分别为18.20和12.46h,表观分布容积(Vd)分别为0.03和0.11L/kg,清除率(CL)均为0.02L/(h·kg)。口灌给药后血药浓度均在12h达到峰值,分布相半衰期(t1/2α)分别为2.84和4.08h,消除相半衰期分别为14.48和12.45h,生物利用度(F)分别为17.02%和18.24%。研究结果表明,温度升高会加快大菱鲆对氟苯尼考的吸收、体内分布和消除,提高口灌方式氟苯尼考利用效率。结合氟苯尼考药效学研究结果,提出给药方案:14℃时,大菱鲆口灌给药剂量25mg/kg BW,每日一次;17℃时,大菱鲆口灌给药剂量22mg/kg BW,每日一次。

关键词(KeyWords)： 氟苯尼考;给药方案;大菱鲆;口灌;药代动力学;温度

Abstract:

Keywords:

基金项目(Foundation): 中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2016HY-ZD1203);; 青岛市2016年海洋经济创新发展示范城市项目;; 泰山产业领军人才工程项目(LJNY2015002)资助~~

作者(Author): 陈钊,罗强,常志强

DOI: 10.16441/j.cnki.hdxb.20180006

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