三种抑制剂对眼点拟微球藻的生长及脂肪酸组成的影响Effects of Three Inhibitors on the Growth and Fatty Acids Composition of Nannochloropsis oculata
张继民,张学成,魏东
摘要(Abstract):
研究了 DCMU、氟哒酮及亚油酸对眼点拟微球藻的生长、总脂含量及不饱和脂肪酸组成的影响。结果表明 :(1)当 DCMU的浓度在 0 .8μmol/ L以上时 ,眼点拟微球藻的生长抑制率达80 % ,其总脂占干重的百分含量减少了近 30 % ;在 1.0 μmol/ L 时 ,EPA占其总脂肪酸的相对百分含量增加了 10 8.6 1%。 (2 )当氟哒酮的浓度在 8.0μmol/ L以上时 ,眼点拟微球藻的生长抑制率为82 .9% ,其总脂占干重的百分含量下降了 2 1% ,而 EPA占其总脂肪酸的相对百分含量增加了6 0 %。(3)当亚油酸的最终浓度在 4 0 0 μmol/ L 以上时 ,眼点拟微球藻的抑制率达 6 4 %以上 ;大于30 0 μmol/ L、4 0 0 μmol/ L 时 ,EPA占其总脂的相对百分含量在 4 .77%以下 ;在 5 0 μmol/ L 以下时 ,EPA占其总脂肪酸的相对百分含量从对照组的 2 0 .96 %下降到 19.4 9%以下。结果表明 ,眼点拟微球藻在养殖中 ,当生物量增加到一定的程度时 ,可以用加入一定浓度的 DCMU或氟哒酮的方法来大幅度提高 EPA的含量。
关键词(KeyWords): 眼点拟微球藻;总脂;EPA;脂肪酸
基金项目(Foundation): 国家“九五”科技攻关项目 ( 96 -CO2 -0 1 -1 0 -8);; 海洋 86 3项目 ( 81 9-0 2 -0 1 )资助
作者(Author): 张继民,张学成,魏东
DOI: 10.16441/j.cnki.hdxb.2002.02.018
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