苗淑彦,韩冬冬,聂琴,苗惠君,张文兵,麦康森

• 1:中国海洋大学水产动物营养与饲料农业部重点实验室海水养殖教育部重点实验室
• 2:扬州大学动物科学与技术学院

摘要(Abstract)：

为研究饲料中糊精替代鱼油对大菱鲆(Scophthalmus maximus)幼鱼代谢及免疫反应的影响,在饲料中蛋白质含量为45%的基础上,配制4组等氮等能的饲料(D1、D2、D3和D4),饲料中可溶性糖含量分别为1.93%、5.80%、15.98%和28.27%,脂肪含量分别为16.48%、12.82%、10.78%和6.64%,其中糊精为糖源,鱼油和大豆卵磷脂为脂肪源。在室内流水系统中养殖大菱鲆幼鱼((8.32±0.06)g),检测大菱鲆幼鱼的血液和肝脏中糖、脂肪和蛋白质代谢及抗氧化指标,试验周期为9周。研究表明:随着饲料中糊精替代鱼油水平的升高,大菱鲆的血糖含量、血浆胰岛素含量、血浆谷草转氨酶(AST)和谷丙转氨酶(ALT)活性(P<0.05)显著升高。其中,D4组AST活性显著高于其余3组(P<0.05),D1组ALT活性显著高于其余3组(P<0.05)。饲料中糊精替代鱼油水平的升高对血浆中总氨基酸、总胆固醇(CL)和甘油三酯(TGs)的含量无显著影响(P>0.05)。随着饲料中糊精含量的不断上升,血浆中溶菌酶、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)呈下降趋势。肝脏中碱性磷酸酶(AKP)和CAT活性以及T-AOC也有一定程度下降(P<0.05),但肝脏溶菌酶活性显著上升(P<0.05)。当饲料可溶性糖含量为15.98%时,肝脏SOD活性最高(P<0.05)。结果表明:饲料中,糊精替代鱼油既影响大菱鲆幼鱼的糖和脂类代谢,又影响其免疫力。在实际生产中,除了关注饲料中糖对鱼体生长的影响,还应关注糖对鱼体代谢及免疫的影响。

关键词(KeyWords)： 大菱鲆;糖;糊精;鱼油;代谢;免疫

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划项目(2014CB138600)资助~~

作者(Author): 苗淑彦,韩冬冬,聂琴,苗惠君,张文兵,麦康森

DOI: 10.16441/j.cnki.hdxb.20150101

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