张红秀,刘晓华,刘伟治

• 1:中国海洋大学海洋生命学院海洋生物遗传学与育种教育部重点实验室

摘要(Abstract)：

为挖掘新型褐藻胶裂解酶，使其开发成为制备褐藻寡糖的工具酶，本研究中表征了来源于海洋细菌Pseudoalteromonas sp. 01的褐藻胶裂解酶AlyPC1。本文作者在生物信息学分析的基础上，对AlyPC1进行了重组表达及酶学性质的研究。序列比对结果显示，AlyPC1是PL7家族第五亚家族的一个褐藻胶裂解酶。酶学性质研究发现：AlyPC1最适反应温度为35℃,将AlyPC1置于5～30℃孵育1 h,仍保留80%以上的酶活；在Tris-HCl缓冲液中，AlyPC1最适反应pH为8.0,当pH在7.5～9.0之间时，酶活保留了90%以上，证明AlyPC1具有良好的温度稳定性以及宽泛的pH耐受性；反应体系中NaCl的含量为200 mmol/L时，AlyPC1的酶活最高；乙二胺四乙酸(EDTA)以及一些金属离子如Mn~(2+)、Zn~(2+)和Ni~(2+)等能够明显抑制AlyPC1的活性。AlyPC1能够降解褐藻胶、polyG以及polyM三种底物，因而说明AlyPC1是一种双功能褐藻胶裂解酶。同时，AlyPC1以内切的形式降解褐藻胶，产生二糖、三糖以及四糖。

关键词(KeyWords)： 褐藻胶裂解酶;褐藻寡糖;内切酶;双功能;稳定性

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2018YFC0311105)资助~~

作者(Author): 张红秀,刘晓华,刘伟治

DOI: 10.16441/j.cnki.hdxb.20220128

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