UV-B辐射对2种海洋微藻膜脂过氧化和脱酯化伤害Studies on Damage of Membrane Lipid Peroxidation and Deesterification Caused by UV-B Radiation in Two Species of Marine Microalgae
王军,肖慧,冯蕾,蔡恒江,唐学玺
摘要(Abstract):
研究海洋生态系统在调节全球变化中的作用,采用生态毒理学的方法研究了UV-B辐射增强对2种海洋微藻膜的损伤及其差异性。结果表明,随着UV-B辐射剂量的增加,叉鞭金藻(Dicrateriasp.)和三角褐指藻(Phaeodactylum tricornu-tum)的光合速率降低。同时,膜相对透性增大,细胞内H2O2含量上升,微粒体膜中磷脂减少,游离脂肪酸增加。表明UV-B辐射增强使2种微藻的膜受到了严重伤害,而且膜伤害的加剧与活性氧的积累和由此引发的膜脂脱酯化有关。2种微藻细胞匀浆中丙二醛(MDA)含量无显著变化,而微粒体膜的MDA含量随着UV-B辐射剂量的加大显著提高。这表明UV-B辐射增强对2种海洋微藻膜的损伤可能是由膜脂过氧化和脱酯化作用共同引起的。
关键词(KeyWords): UV-B辐射增强;膜脂过氧化;脱酯化;海洋微藻
基金项目(Foundation): 国家自然科学基金项目(30270258);; 新世纪优秀人才支持计划奖励基金项目(NCET-05-0597)资助
作者(Author): 王军,肖慧,冯蕾,蔡恒江,唐学玺
DOI: 10.16441/j.cnki.hdxb.2006.05.017
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