于广利,管华诗,Robert J.Linhardt,Jawed Fareed

• 1:青岛海洋大学海洋药物与食品研究所!青岛
• 2:266003
• 3:青岛海洋大学海洋药物与食品研究所!青岛
• 4:266003

摘要(Abstract)：

研究肝素酶 (来源于 Flavobacterum heparinase,EC4 .2 .2 .7)对人工合成的且含有与抗凝血酶 特定结合位点的肝素五糖 (SHP)的酶解作用 ,并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱 (SAX- HPL C)对酶解混合物进行分离 ,利用质谱 (ESIMS)和核磁共振波谱 (1H- NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明 ,这种被作为肝素反向试剂的肝素酶 可水解人工所合成的肝素五糖 ,从而使之丧失抗 Xa因子活性。

关键词(KeyWords)： 肝素酶Ⅰ(Heparinase Ⅰ);合成肝素五糖(SHP);抗Xa因子活性

Abstract:

Keywords:

基金项目(Foundation): 美国国立健康研究学会NIH(GM380 6 0 )基金资助&&

作者(Author): 于广利,管华诗,Robert J.Linhardt,Jawed Fareed

DOI: 10.16441/j.cnki.hdxb.2001.04.012

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