壬基酚对栉孔扇贝组织抗氧化酶活性的影响Effect of Nonylphenol on Anti-Oxidant Enzymes of Chlamys farreri
高永刚,李正炎
摘要(Abstract):
研究不同浓度的壬基酚(0.05,0.10,0.20,0.40,0.80,1.60mg/L)对扇贝组织超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响。试验进行6h,24h和48h后,分别测定扇贝鳃和消化盲囊组织中2种酶的活性。结果显示:①鳃组织中,0.05,0.10和0.20mg/L处理组中的SOD和CAT活性在试验6h后被轻度激活,24h和48h后出现抑制;0.40,0.80和1.60mg/L处理组的酶活性在整个试验过程中均被抑制。②消化盲囊中,0.05和0.10mg/L处理组中的SOD和CAT活性在试验6h后被轻度激活,24h和48h后被抑制;0.40,0.80和1.60mg/L处理组在整个试验过程中均被抑制。③扇贝消化盲囊组织中的SOD和CAT活性均高于鳃组织,对壬基酚的敏感性也高于后者。④扇贝消化盲囊和鳃组织中的SOD和CAT活性对壬基酚的反应变化趋势相似,二者结合,可以作为壬基酚污染的生物标志物。
关键词(KeyWords): 壬基酚;栉孔扇贝;超氧化物歧化酶;过氧化氢酶;生物标志物
基金项目(Foundation): 国家自然科学基金项目(40306019);; 山东省环境保护科技项目(2004049)资助
作者(Author): 高永刚,李正炎
DOI: 10.16441/j.cnki.hdxb.2006.s1.026
参考文献(References):
- [1]Craig D,Brown E.Effects of sewage effluent and ethynyl oestradiolupon molecular markers of oestrogenic exposure,maturation and re-productive success in the sand goby(Pomatoschistus minutus,Pallas)[J].Aquatic Toxicology,2003,62:119-134.
- [2]Wiston G.Oxidants and antioxidants in aquatic animals[J].CompBiochem Physiol,1991,100:173-176.
- [3]Stephensen E,Svavarsson J,Sturve J,et al.Biochemical indicators ofpollution exposure in shorthorn sculpin,caught in four harbours on thesouthwest coast of Icaland[J].Aquat Toxicol,2000,48:431-442.
- [4]王宏,沈英娃.烷基酚聚氧乙烯醚类物质的环境雌激素效应[J].中国环境科学,1999,19(5):427-431.
- [5]Ekelund,Bergman,Granmo,et al.Bioaccumulation of 4-nonylphenolin marine animals A re-evaluation[J].Environmental Pollution,1990,64:107-120.
- [6]Ferguson,Lee Iden,Charles,et al.Analysis of nonylphenol andnonylphenol ethoxylates in environmental samples by mixed-modehigh-performance liquid chromatography-electrospray mass spectrome-try[J].Journal of Chromatography A,2001,938:79-91.
- [7]徐镜波,袁小凡,郎佩贞.过氧化氢酶活性及活性抑制的紫外分光光度法测定[J].环境化学,1997,16(1):73-76.
- [8]张宏,谭竹钧.四种邻苯三酚自氧化法测定超氢化物歧化酶活性方法的比较[J].内蒙古大学学报:自然科学版,2002,33(6):677-681.
- [9]游学军,郑永华.对氯硝基苯对草鱼肝胰脏过氧化氢酶活性的影响[J].西南农业学报,2001,14(4):79-82.
- [10]冯涛,郑微云,洪万树,等.苯并芘对大弹涂鱼肝脏抗氧化酶活性影响的初步研究[J].应用生态学报,2001,12(3):422-424.
- [11]李康,周中良,王明山,等.苯并芘对鲫鱼(Carassius auratus)肝脏抗氧化酶的影响[J].应用与环境生物学报,2003,10(1):88-91.
- [12]Klump D W,Humphrey C,Hong H,et al.Toxic contaminants andtheir biological effects in coastal waters of Xiamen,ChinaⅡ:biomarkers and embryo malformation rates as indicators of pollutionstress in fish[J].Mar Pollut Bull,2002,44:761-769.
- [13]周永欣.水生生物毒性试验方法[M].北京:农业出版社,1989.
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