徐武杰,潘鲁青,岳峰,李健

• 1:中国海洋大学海水养殖教育部重点实验室
• 2:中国水产科学研究院黄海水产研究所

摘要(Abstract)：

以三疣梭子蟹(Portunus trituberculatus)为研究对象,研究氨氮胁迫对中肠腺、胃、肠组织消化酶活力的影响。实验设计对照组(自然海水)、1、5、20 mg/L处理组,分别于氨氮胁迫后0、6、12、244、8 h取样进行消化酶活力的测定。结果表明:氨氮胁迫对三疣梭子蟹类胰蛋白酶、胃蛋白酶、脂肪酶和淀粉酶活力影响显著(P<0.05),而对照组无明显变化。各处理组3种消化器官中类胰蛋白酶、胃蛋白酶活力在48 h内呈峰值变化,其中3种消化器官中类胰蛋白酶活力在48 h时恢复至对照组水平,而中肠腺胃蛋白酶活力在24 h后趋于稳定,且与氨氮浓度呈明显正相关,胃和肠的胃蛋白酶活力分别于48和24 h时恢复至对照组水平。各处理组中肠腺和胃的脂肪酶活力在24 h内均呈现明显的峰值变化,24 h后中肠腺中脂肪酶活力趋于稳定,且与氨氮浓度呈明显正相关,而胃中脂肪酶活力逐渐下降,至48 h时恢复至对照组水平。各处理组中肠腺和肠的淀粉酶活力在12 h内呈逐渐下降趋势,12 h后趋于稳定,且与氨氮浓度呈明显负相关;各处理组胃的淀粉酶活力呈现先升高后降低趋势,24 h后趋于稳定。同时在氨氮胁迫48 h时,对中肠腺和胃的胃蛋白酶、脂肪酶活力均表现为明显的诱导作用,而对3种消化器官中淀粉酶活力表现为明显的抑制作用。由此说明,三疣梭子蟹在氨氮胁迫下中肠腺中胃蛋白酶、脂肪酶活力和3种消化器官中淀粉酶活力可作为消化吸收能力的评价指标。

关键词(KeyWords)： 氨氮胁迫;三疣梭子蟹;消化酶活力

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展规划项目(2006AA10A406);; 教育部新世纪优秀人才支持计划项目(NCET-06-0597)资助

作者(Author): 徐武杰,潘鲁青,岳峰,李健

DOI: 10.16441/j.cnki.hdxb.2011.06.006

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