王诗媛,苏海鹏,毛相朝,胡阳

• 1:中国海洋大学食品科学与工程学院
• 2:海洋食品加工与安全控制全国重点实验室
• 3:青岛海洋科技中心海洋药物与生物制品功能实验室
• 4:青岛市食品生物技术重点实验室

摘要(Abstract)：

为获得高反应温度的壳聚糖酶，本文挖掘了来源于δ变形细菌的壳聚糖酶基因(GenBank:MBN2801850.1)并将其在大肠埃希氏菌(Escherichia coli)中成功异源表达得到新的壳聚糖酶Csn(Db)-1-WSY(Csn1)。Csn1是属于GH8家族的壳聚糖酶，蛋白分子量大小约为52 kDa,比酶活为135.81 U/mg,是典型的内切型壳聚糖酶，其在60℃、pH=10.0的Gly-NaOH缓冲液中表现出最高活性。Csn1的米氏常数K_m为1.30 mmol/L,显示其与底物较好的亲和性。在5 mmol/L Cu~(2+)溶液中Csn1的活性增强至初始的131.36%;而Fe~(2+)、Co~(2+)、Mn~(2+)等金属离子均对Csn1表现出活性抑制作用。Csn1水解壳聚糖的主要产物为(GlcN)_2至(GlcN)_4。研究结果表明，Csn1可为工业制备壳寡糖提供有效的生产工具酶。

关键词(KeyWords)： 内切型壳聚糖酶;壳寡糖;酶学性质;壳聚糖

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(32201966);; 中央高校基本科研业务费专项(202213027)资助~~

作者(Author): 王诗媛,苏海鹏,毛相朝,胡阳

DOI: 10.16441/j.cnki.hdxb.20230336

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