樊廷俊,魏云波,徐晓辉,孙爱,姜国建

• 1:中国海洋大学海洋生命学院海洋生物系

摘要(Abstract)：

为建立褐点石斑鱼鳍、心脏和鳔组织细胞系,本文利用不同培养液和培养温度对褐点石斑鱼鳍、心脏和鳔组织细胞进行了原代培养和传代培养。实验结果显示,在24℃培养于含有羧甲基壳寡糖、碱性成纤维样生长因子、I型胰岛素样生长因子及20%胎牛血清的DMEM/F12培养液(pH=7.2)中的3种细胞,均为成纤维样细胞,其生长分裂状态最佳,可以持续稳定传代。第60代褐点石斑鱼鳍、心脏和鳔细胞的群体倍增时间分别为50.6 h、40.3 h和43.3 h,其特征性染色体数目均为48条。目前鳍、心脏和鳔细胞已分别传至第90代、第70代和第75代,已成功建立了3种细胞的连续性细胞系,为鱼类病毒与宿主细胞相互作用机制等鱼类病毒学基础研究,以及鱼类病毒的分离、鉴定、繁殖及病毒疫苗研制等奠定了基础。

关键词(KeyWords)： 褐点石斑鱼;鳍细胞;心脏细胞;鳔细胞;细胞系

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2006AA10A4012006AA09Z406)资助

作者(Author): 樊廷俊,魏云波,徐晓辉,孙爱,姜国建

DOI: 10.16441/j.cnki.hdxb.2009.05.026

参考文献(References)：

• [1]Villena AJ.Applications and needs of fishandshellfishcell culturefordisease control[J].Reviews in Fish Biology and Fisheries,2003,13:111-140.
• [2]Fryer J L,Lannan C N.Three decades of fish cell culture:Acurrentlisting of cell lines derivedfromfishes[J].J Tissue Culture Methods,1994,16(2):87-94.
• [3]Chen S L,Ren G C,Sha Z X,et al.Establishment of a continuousembryonic cell line fromJapanese flounderParalichthys olivaceusforvirus isolation[J].Dis Aquat Org,2004,60:241-246.
• [4]Chen S L,Ren G C,Sha Z X,et al.Development and characteriza-tion of a continuous embryonic cell line fromturbot(Scophthal musmaxi mus)[J].Aquaculture,2005,249:63-68.
• [5]樊廷俊,耿晓芬,丛日山,等.大菱鲆鳍细胞系的建立[J].中国海洋大学学报:自然科学版,2007,37(5):759-766.
• [6]Chen S L,Sha Z X,Ye HQ,et al.Pluripotency andchi mera compe-tence of an embryonic stemcell line fromthe sea perch(Lateolabraxjaponicus)[J].Mar Biotechnol(NY),2007,9(1):82-91.
• [7]I majoh M,Ikawa T,Oshi ma S.Characterization of a newfibroblastcell line froma tail fin of red sea bream,Pagrus major,and phyloge-netic relationships of a recent RSI Visolate in Japan[J].Virus Res,2007,126(1-2):45-52.
• [8]Qin Q W,Wu T H,Jia T L,et al.Development and characterizationof a new tropical marine fish cell line from grouper,Epinepheluscoioidessusceptible toiridovirus and nodavirus[J].J Virol Methods,2006,131(1):58-64.
• [9]Wen C M,Lee C W,Wang C S,et al.Development of two cell linesfromEpinephelus coioidesbrain tissue for characterization of betano-davirus and megalocytivirus infectivity and propagation[J].Aquacul-ture,2008,278:14-21.
• [10]Lai Y S,John J A,Lin C H,et al.Establishment of cell lines froma tropical grouper,Epinephelus awoara(Temminck&Schlegel),and their susceptibility to grouper irido-and nodaviruses[J].J FishDis,2003,26(1):31-42.
• [11]Zhou G Z,Li Z Q,Yuan X P,et al.Establishment,characteriza-tion,and virus susceptibility of a new marine cell line fromred spot-ted grouper(Epinephelus akaara)[J].Mar Biotechnol(NY),2007,9(3):370-376.
• [12]Qin Q W,Shi C,Gin K Y,et al.Antigenic characterization of amarine fish iridovirus from grouper,Epinephelus spp[J].J VirolMethods,2002,106:89-96.
• [13]Gibson-Kueh S,Ngoh-Li m G H,Netto P,et al.Asystemiciridovi-ral disease in mullet,Mugil cephalus L.,and tiger grouper,Epinephelus fuscoguttatusForsskal:a first report and study[J].JFish Dis,2004,27:693-699.
• [14]廖经球,尹绍武,陈国华,等.褐点石斑鱼的核型研究[J].水产科学,2006,11(23):567-569.

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