侯媛媛,陈慕雁

• 1:海水养殖教育部重点实验室(中国海洋大学)

摘要(Abstract)：

本文以仿刺参(Apostichopus japonicus)肠道组织为研究材料,利用HE染色、米利根染色和免疫组化技术对其组织学结构及神经定位进行了观察和分析,初步探讨了仿刺参肠道的神经分布。HE染色结果表明,仿刺参肠壁由外向内分别为:体腔上皮层、肌肉层、内部结缔组织层和肠腔上皮层;和后肠相比,其中肠内壁常形成高而窄,且方向单一的褶皱,在结构上扩大了吸收面积,与其主要起消化吸收作用这一功能一致。米利根染色表明肠壁的纵向肌肉、肠腔上皮层和体腔上皮层均有神经成分存在,棘皮动物神经特异性抗体1E11的定位研究结果同样显示其免疫活性广泛而强烈,阳性位点集中于体腔上皮层、肌肉层和内部结缔组织层,且在肌肉层中表达最强,推测其可能参与肠道的再生过程及长期夏眠萎缩后的肠道快速修复过程。本研究为仿刺参神经生物学的研究提供了理论支持,同时也为仿刺参特殊生理机制的研究提供神经学参考资料。

关键词(KeyWords)： 仿刺参;肠道;组织学;免疫组化;神经生物学

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41676124)资助~~

作者(Author): 侯媛媛,陈慕雁

DOI: 10.16441/j.cnki.hdxb.20180060

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